Promega dna ladder molecular weight markers, 1kb dna ladder. Promega dna ladder molecular weight markers, 100bp dna ladder. Step ladders are not intended for concentration estimations. These ladders consist of equimolar mixtures of dna fragments for determining the mass of unknown dna samples on gels in the low and high molecular weight ranges. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. The dna includes fragments ranging from 25010,000 base pairs. The benchtop dna markers are supplied in a stabilizing solution of 1x blueorange loading dye, making the markers ready to load. Two internal features facilitate band identification.
The benchtop 100bp dna ladder has 11 fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. Generuler 1 kb plus dna ladder thermo fisher scientific. The product is supplied in a solution of 10 mm trishcl, ph 8. The 100bp dna ladder has eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp. The benchtop 1kb dna ladder has bluntended fragments with sizes ranging from 250bp to 10,000bp. Your search returned 25 dna ladders ladders and markers across 1 supplier. The ladders are not intended for use in quantitative analysis. Thermo scientific generuler 1 kb dna ladder is designed for sizing and approximate quantification of wide range doublestranded dna on agarose gel. Dna marker blue bp 1kb dna ladder readytouse dna marker bp 1kb dna ladder is designed for use as molecular weight standard for agarose gel electrophoresis features.
Anteil toter zellen in einer agspopulation nach transfektion mit pegfpn1. Is it ok to use dna molecular weight marker in rna. Highranger 1kb dna ladder 300bp 0bp size determination of digested dna. I preferred this product because it has premixed dye thus its ready to use and lasts a long time. Promega 1kb dna ladder molecular weight marker 1kb dna. A highintensity 500bp band stands out at the lowest segment of the ladder free 18006325227 fax.
Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. The dna 1 kb ladder is suitable for size determination of doublestranded dna using dna electrophoresis. Free sample available, use link below to request free sample highranger plus 100bp dna ladder 100bp 0bp analyzing dna over a wide range of sizes. I am guessing the two bands showing up in the lanes with samples are the 28s and 18s rna bands but they dont. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed with 1ul of blueorange 6x loading dye. Dna mass ladders are specifically created for quantitative estimation of dna mass in gels. A rapid protocol to isolate highquality plasmid dna, using a silica membrane column, in less than 10 minutes, depending on the number of samples. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and 1kb bands. Antibody and t cell responses induced in chickens immunized. The ladder is composed of fourteen chromatographypurified individual dna fragments in base pairs. Generuler 1 kb dna ladder thermo fisher scientific. Promega dna ladder molecular weight markers, 100bp dna. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. For example, step ladder dna fragments could be in increments of 100bp from 100bp all the way up to 4000bp.
A highintensity 500bp band stands out at the lowest segment of the ladder dna ladders are ladders with defined sizes. Eleven fragments that range in size from 100bp to 1,000bp in. Tridye 1 kb dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. Dna ladders have defined sizes, and are not intended for use in quantitative analysis. View more information about our dna stains technology. How can i prepare working solution with 1 kb dna ladder. Download a pdf containing pricing for our full product list. Forty bluntended dna fragments ranging from 100bp to 4,000bp in 100bp increments. Example of some subtypes refer to table 2 for strain names assayed. These standards contain predetermined fragment or protein sizes and concentrations. Molecular weight markers, or ladders, are a set of standards that are used for determining the approximate size of a protein or a nucleic acid fragment run on an electrophoresis gel.
All bands except the 800bp band are of equal intensity. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis. The 500bp fragment is present at increased intensity for easy identification. Endogenous expression of an antitar aptamer reduces hiv1. Promega 1kb dna ladder molecular weight marker 1kb dna ladder. Dna step ladders contain dna bands of defined sizes and equal intensity with fragment lengths at defined increments. The 1k and 3k bands have increased intensity to serve as reference points. Each ladder is provided with a tube of 6x blueorange loading dye. The doublestranded dna is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated to 10 rnm trishci ph 8. If samples need to be diluted, use te or other buffer of minimal ionic strength. Fullranger 100bp dna ladder 100bp 5000bp good range for small and large cloning applications. The quickload purple 1 kb dna ladder is not intended for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size.
Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. The approximate mass of dna in each band is provided 0. Eleven fragments that range in size from 100bp to 1,000bp in 100bp increments plus a band at 1,500bp. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. I used promega 1 kb ladder to check the product size of my pcr product. This ladder consists of bands containing 18 repeats. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible.
This marker can be used with either agarose or polyacrylamide gels. Thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. For alternative plasmids with fluorescent tags, try plasmids from doug. Quickload purple 1 kb dna ladder is stable for at least 6 months at 25c. The ladder is dephosphorylated and ready for 5 endlabeling with radioisotopes using t4 polynucleotide kinase, allowing visualization by autoradiography. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. The 1kb dna ladder marker consists of nine double 1 kb dna ladder cat. Search results for 1 kb dna ladder at sigmaaldrich. A broad spectrum, onestep reversetranscription pcr amplification.
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